“不繁”,强调制度的简洁可行,突出针对性和指导性。“不繁”之要,是以必要的规则,达致有效、严密的约束。若条文冗长空洞、脱离实践,非但不能发挥制度的规范作用,还会因难以把握、让执行者无所适从而损害制度威信。习近平总书记强调“不管建立和完善什么制度,都要本着于法周延、于事简便的原则”,指出了“密而不繁”的主要内涵。其中,于法周延的核心在“密”,织密制度之网,实现系统集成、协同高效;于事简便的关键在“简”,剔除冗余繁琐,确保制度立得住、行得通、真管用。
In 2010, ONT combined two technologies addressing each of the main outstanding problems. The first was an engineered nanopore developed in collaboration with Bayley’s lab that could discriminate between individual DNA bases, solving the resolution issue. The second was a trick to slow the DNA down to detectable speeds, using the familiar DNA polymerase enzyme. Mark Akeson’s lab at UC Santa Cruz had identified a specific polymerase from the bacterial virus ɸ29 that replicated DNA at an ideal speed for detection via nanopore. Template DNA strands were replicated just before entering the nanopore, passing through slowly enough for individual bases’ effect on the electrical current to be detectable and allowing the DNA sequence to be read one base at a time.
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